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Test Code TDP Thiamine (Vitamin B1), Whole Blood

Important Note

This test requires whole blood, protected from light.  DO NOT SPIN!!! Must pour whole blood into amber tube and freeze within 24 hours. Protect original tube from light during transport if you do not have an amber vial. 

Useful For

Assessment of thiamine deficiency

 

Measuring thiamine levels in patients with behavioral changes, eye signs, gait disturbances, delirium, and encephalopathy; or in patients with questionable nutritional status, especially those who appear at risk and who also are being given insulin for hyperglycemia

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

Thiamin (Vitamin B1), WB

Specimen Type

Whole Blood EDTA


Shipping Instructions


Ship specimen in amber vial or tube to protect from light.



Specimen Required


Patient Preparation:

1. Patient should fast overnight (12-14 hours); infants should have specimen collected just before next feeding. Water can be taken as needed.

2. For 12 hours before specimen collection, patient should not take vitamin supplements.

Supplies: Amber Frosted Tube, 5 mL (T915)

Collection Container/Tube: Lavender top (EDTA)

Submission Container/Tube: Amber vial

Specimen Volume: 4 mL

Collection Instructions:

1. Invert 8 to 10 times to mix blood.

2. Transfer whole blood into amber vial or tube and freeze within 24 hours of collection.


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood EDTA Frozen 28 days LIGHT PROTECTED

Reject Due To

Gross lipemia Reject
Glass vial
Clotted specimen		 Reject

Reference Values

70-180 nmol/L

Day(s) Performed

Monday through Friday

Report Available

3 to 6 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

84425

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TDP Thiamin (Vitamin B1), WB 32554-8

 

Result ID Test Result Name Result LOINC Value
85753 Thiamin (Vitamin B1), WB 32554-8

Forms

If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.