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Test Code MISC PML-RARA Translocation (t[15;17]), Qualitative

Additional Codes

Supplemental Test Code
5706

Methodology

Reverse Transcription-Polymerase Chain Reaction (RT-PCR) amplification using primers specific for the S- and L-forms of the PML-RARA fusion followed by capillary electrophoresis for size separation.
(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)

Literature Reference:

Miller WH, et al. RT-PCR for the rearranged RAR alpha clarifies diagnosis and detects minimal residual disease in APL. Proc Natl Acad Sci USA 1992;89:2694-8.

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Specimen Requirements

Call Barnes-Jewish Hospital Molecular Diagnostics Laboratory at 314-454-8685 for collection instructions.

 

Forms: Molecular Diagnostics Laboratory Request for DNA Studies-Oncology in Special Instructions.

 

Submit only 1 of the following specimens:

 

Specimen Type: Bone Marrow

Container/Tube: Lavender top (EDTA)

Specimen Volume: 1-2 mL

Specimen Minimum Volume: 1 mL

Collection Instructions: Invert several times to mix bone marrow. Clotted bone marrow is not acceptable.

Additional Information: If specimen is not couriered, send Express Mail or equivalent for receipt Monday through Friday to avoid specimen degradation.

 

Specimen Type: Tissue, formalin-fixed, paraffin-embedded (FFPE)

Container/Tube: Sterile, plastic microcentrifuge tube

Specimen Volume: Twenty, 10 micron sections

 

Specimen Type: Tissue, fresh frozen

Container/Tube: Sterile, plastic container

Specimen Volume: 10 mm3

 

Specimen Type: Blood

Container/Tube: Lavender top (EDTA)

Specimen Volume: Full tube

Specimen Minimum Volume: 1 mL

Collection Instructions: Invert several times to mix blood. Clotted blood is not acceptable.

Additional Information: If specimen is not couriered, send Express Mail or equivalent for receipt Monday through Friday to avoid analyte degradation.

Specimen Transport Temperature

Ambient/Refrigerate NO/Frozen NO-Blood, Bone Marrow, Paraffin-Embedded Tissue

Dry Ice-Fresh Tissue

Reference Values

An interpretive report will be provided.

Literature Reference:

Gallagher R, Willman C, Slack J, et al. Association of PML-RAR alpha fusion mRNA type with pretreatment hematologic characteristics but not treatment outcome in acute promyelocytic leukemia: an intergroup molecular study. Blood 15 August 1997;90:1656-63.

Day(s) Test Set Up

Monday and Wednesday
Turnaround Time:
STAT: Director approval required
Routine: 3 to 5 business days

Test Classification and CPT Coding

81315 

Additional Information

For BJH Laboratory Use Only

Alternate Tube:

1 yellow-top ACD (solution A or B) tube

Analyte Stability:

Blood and bone marrow are stable up to 4 hours at ambient temperature
FFPE tissue stable indefinitely
Fresh-frozen tissue stable at -20o C

Laboratory Processing Instructions:
Test performed in BJH Molecular Diagnostics.

Note: If specimen is received after hours (1700 weekdays), place in designated refrigerator (4° C) in North Campus Laboratory.

Analyte stability for storage of specimens received before DNA or RNA processing:

Bone Marrow:
DNA analysis-specimen can be stored up to 96 hours at 4° C

RNA analysis-specimen can be stored up to 4 hours at 4° C

Peripheral Blood:
DNA analysis-specimen can be stored up to 96 hours at 4° C

RNA analysis-specimen can be stored up to 4 hours at 4° C

Fresh Tissue Biopsy:
DNA and RNA analysis-specimen can be stored up to 4 hours at 4° C

FFPE:
DNA and RNA analysis-specimen can be stored at 23° C indefinitely

Frozen Tissue:
DNA analysis-store specimens at -20° C

RNA analysis-store specimens at -70° C

Special Instructions