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Test Code LAB9781 STR Enrichment Identity Testing, Separated for CD15

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Methodology

Short tandem repeat (STR) polymorphic DNA markers are amplified by polymerase chain reaction (PCR) using the Promega PowerPlex 16 HS System, labeled with fluorescent markers and used to distinguish patient and donor cells as different on a Capillary Electrophoresis Fluorescence Detection instrument.

STR markers used in analysis include: D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, Amelogenin, vWA, D8S1179, TPOX, and FGA. (PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)

References

PowerPlex16 HS System Technical Manual. Promega Corporation, 6/2012 revision

 

Van Deerlin VMD. Identity testing: Applications of DNA typing Anatomic Pathology. In Diagnostic Molecular Pathology, Leonard DGB, editor; 2002 Elsevier Science, Philadelphia, PA.

Specimen Requirements

Call Barnes-Jewish Hospital Molecular Diagnostics Laboratory at 314-454-8685 for collection instructions.

Form: Molecular Diagnostics Requisition

 

Couriered specimen must arrive within 4 hours of collection Monday through Friday only.

 

Specimen Type: Whole blood

Container/Tube: Pink top (EDTA)

Specimen Volume: Full tube

Specimen Minimum Volume:

3 mL of EDTA whole blood 


Collection Instructions: Invert several times to mix blood. Clotted blood is not acceptable.

Additional Information: 

1. Specimen cannot be frozen.

2. If specimen is not couriered, send Express Mail or equivalent for receipt Monday through Friday to avoid specimen degradation.

Day(s) Test Set Up

Monday and Wednesday
Turnaround Time:
STAT:  Not available
Routine: 5 to 7 business days

Reference Values

An interpretative report will be provided.

Literature Reference:

Au WY, Chan EC, Lie AKW, et al. Poor engraftment after allogeneic bone marrow transplantation:  role of chimerism analysis in treatment and outcome. Ann Hematol 2003;82:410-15.

Test Classification and CPT Coding

81268 

Additional Information

For BJH Laboratory Use Only
Analyte Stability:

Blood is stable 0 to 4 hours at ambient temperature, 72 hours at 4C

Laboratory Processing Instructions:

Enrichment performed in BJH Flow Cytometry; test performed in BJH Molecular Diagnostics.

Specimen Transport Temperature

Ambient/Refrigerate/Frozen NO