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Test Code LAB928 Paroxysmal Nocturnal Hemoglobinuria (PNH) Profile

Methodology

Flow Cytometry

Includes CD59-RBC membrane inhibitor of reactive lysis (MIRL), CD59-WBC MIRL, and fluorescent-labeled inactive toxin aerolysis (FLAER).

Literature References:

1. Krauss JS. Laboratory diagnosis of paroxysmal nocturnal hemoglobinuria. annals of clinical and laboratory science 2003;33:401-6.
2. Pui JC, Schreitzenmeir R, Moore, JS. Flow cytometric diagnosis of paroxysmal nocturnal hemoglobinuria, Purdue Education Flow Vol 3.

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Specimen Requirements

Specimen must arrive within 48 hours of draw. Specimen must arrive by 1800 on Friday and 1 day before a holiday.

 

EDTA and sodium heparin whole blood are required.

 

Specimen Type: Whole blood

Container/Tube: Pink/Lavender top (EDTA)

Specimen Volume: Full tube

Specimen Minimum Volume: 1 mL or 5 mL if 1 tube is submitted (Testing can be performed on either EDTA or sodium heparin whole blood if only 1 tube with is submitted with sufficient volume.)

 

Specimen Type: Whole blood

Container/Tube: 10-mL dark-green top (sodium heparin)

Specimen Volume: Full tube

Specimen Minimum Volume: 5 mL or 5 mL if 1 tube is submitted (Testing can be performed on either EDTA or sodium heparin whole blood if only 1 tube with is submitted with sufficient volume.)

Specimen Transport Temperature

Ambient

Day(s) Test Set Up

Monday through Friday

Cut-off time: 1800

Turnaround Time:
STAT: not available
Routine: results available within 48 hours.

The final interpretation will be determined by the hematopathologist.  A surgical pathology report will be provided.

Test Classification and CPT Coding

88184-Flow cytometry, first marker
88185 x 2-Flow cytometry, additional markers

Additional professional charges may apply.

Additional Information

For BJH Laboratory Use Only

Alternate Tube:

1 dark green-top (lithium heparin) tube

Analyte Stability:

CD59-RBC is stable for 48 hours at ambient room temperature or 1 week if specimen is refrigerated.

FLAER is stable for 48 hours at ambient room temperature or 4 days if specimen is refrigerated.

CD59-WBC is stable for 48 hours at ambient room temperature and refrigerated.

Laboratory Processing Instructions:

Test performed in BJH Flow Cytometry.