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HelpTest Code LAB6464 Extractable Nuclear Antigens (ENA) Screen, Serum
Methodology
Multiplex Flow Immunoassay
Testing Algorithm:
If ENA screen is positive, Anti-RNP Antibody; Anti-Sm Antibody, Anti-SSA/Ro Antibody; Anti-SSB/La Antibody; Anti-Scl-70 Antibody; and Anti-Jo-1 Antibody will be performed at an additional charge.
Literature Reference:
Package Insert: BioPlex 2200 ANA Screen kit. BioRad Laboratories, Inc. 03/2012
Performing Laboratory
Barnes-Jewish Hospital Laboratory
Specimen Requirements
Specimen Type: Serum
Container/Tube: Plain red top, Red/grey SST or Gold SST
Specimen Volume: Full tube
Specimen Transport Temperature
Ambient
Reference Values
Negative
Positive screens will be reflexed to specific testing for antibodies against the following antigens: Jo-1 Ab, RNP Ab, Scl-70 Ab, Sm Ab, SS-A/Ro Ab and SS-B/La Ab. Further testing for dsDNA, Centromere, and Ribosomal P antibodies is suggested in patient with a positive screen and negative specific antibodies.
Literature References:
Package Insert: BioPlex 2200 ANA Screen kit. BioRad Laboratories, Inc. 03/2012
Day(s) Test Set Up
Monday through Sunday
Cut-off time: 1000
Turnaround Time:
STAT: not available
Routine: results available the day of testing
Test Classification and CPT Coding
86235-ENA antibody
86235 x 6-ENA antibody (if appropriate)
Additional Information
For BJH Laboratory Use Only
Analyte Stability:
See Analyte Stability Chart
Laboratory Processing Instructions:
Test performed in BJH Core Lab. Processed by automated line and aliquot stored refrigerated for batch analysis. Completed specimens are stored refrigerated for 5 days.
LOINC Code Information
| Name | LOINC Code |
| Extractable Nuclear Antigen Screen | 47383-5 |
| Jo-1 Antibody | 5234-0 |
| RNP Antibody | 51928-0 |
| Scl-70 Antibody | 5348-8 |
| Smith Antibody | 43182-5 |
| Sjogren's Syndrome A Antibody | 33569-5 |
| Sjogren's Syndrome B Antibody | 45142-7 |