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HelpTest Code LAB5740 Immune Competence Assessment
Methodology
| Profile Information: | |
| CD3 | CD8 Absolute |
| CD3 Absolute | CD19 |
| CD4 | CD19 Absolute |
| CD4 Absolute | CD16+CD56 |
| CD4/CD8 Ratio | CD16+CD56 Absolute |
| CD8 | |
Method Name:
Flow Cytometry-Single Platform
Literature References:
1. U.S. Department of Health and Human Services, Public Health Service, CDC. Flow cytometric immunophenotyping quality assurance and quality procedures for CD4+T-lymphocyte determinations. 04/1993.
2. NCCLS tentative guidelines. Clinical application of flow cytometry: quality assurance and immunophenotyping of peripheral blood lymphocytes. H42T 12:6.
3. MMWR Recommendation and Reports, Guidelines for performing single-platform absolute CD4 determination with CD45 gating for persons infected human immunodeficiency virus, January 21, 2003.
Performing Laboratory
Barnes-Jewish Hospital Laboratory
Specimen Requirements
Specimen must arrive within 24 hours of draw. Specimen must arrive by 1600 on Friday and 1 day before a holiday.
Specimen Type: Whole blood
Container/Tube: Lavender top (EDTA)
Specimen Volume: Full tube
Specimen Minimum Volume: 1 mL
Additional Information: Specimen cannot be refrigerated.
Specimen Transport Temperature
Ambient/Refrigerate NO/Frozen NO
Reference Values
CD4/CD8 RATIO
0.9-4.4
| Total Cells (CD3) |
Helper Cells (CD4) |
Cytotoxic T-Cells (CD8) |
B Cells (CD19) |
Natural Killer CD16+/CD56+ |
|
| 0-3 Months | |||||
| Percentage: | 53%-84% | 35%-64% | 12%-28 | 6%-32% | 4%-18% |
| Absolute (Cells/mcL): | 2500-5500 | 1600-4000 | 560-1700 | 300-2000 | 170-1100 |
| 3-6 Months | |||||
| Percentage: | 51%-77% | 35%-56% | 12%-23% | 11%-41% | 3%-14% |
| Absolute (Cells/mcL): | 2500-5500 | 1800-4000 | 590-1600 | 430-3000 | 170-830 |
| 6-12 Months | |||||
| Percentage: | 49%-76% | 31%-56% | 12%-24% | 14%-37% | 3%-15% |
| Absolute (Cells/mcL): | 1900-5900 | 1400-4300 | 500-1700 | 610-2600 | 160-950 |
| 1-2 Years | |||||
| Percentage: | 53%-75% | 32%-51% | 14%-30% | 16%-35% | 3%-15% |
| Absolute (Cells/mcL): | 2100-6200 | 1300-3400 | 620-2000 | 720-2600 | 180-920 |
| 2-6 Years | |||||
| Percentage: | 53%-75% | 28%-47% | 16%-30% | 14%-33% | 4%-17% |
| Absolute (Cells/mcL): | 1400-3700 | 700-2200 | 490-1300 | 370-1400 | 130-720 |
| 6-12 Years | |||||
| Percentage: | 60%-76% | 31%-47% | 18%-35% | 13%-27% | 4%-17% |
| Absolute (Cells/mcL): | 1200-2600 | 650-1500 | 370-1100 | 270-860 | 100-480 |
| 12-17 Years | |||||
| Percentage: | 56%-84% | 31%-52% | 18%-35% | 6%-23% | 3%-22% |
| Absolute (Cells/mcL): | 1000-2200 | 530-1300 | 330-920 | 110-570 | 67-1134 |
| Adult | |||||
| Percentage: | 60%-88% | 31%-64% | 12%-40% | 6%-25% | 5%-25% |
| Absolute (Cells/mcL): | 661-1963 | 365-1294 | 187-781 | 86-488 | 76-467 |
Day(s) Test Set Up
Monday through Friday
Cut-off time: 2100 Monday through Thursday; 1600 Friday and eve before a holiday
Turnaround Time:
STAT: not available
Routine: 48 hours
Test Classification and CPT Coding
86355-B-cells, total count
86357-Natural killer cells, total count
86359-T-cells, total count
86360-T-cells CD4+CD8 with ratio
Additional Information
For BJH Laboratory Use Only
Alternate Tube:
1 dark green-top (sodium heparin) tube
Analyte Stability:
Specimen cannot be refrigerated. Specimen must be processed within 48 hours of draw and kept at ambient temperature.
Laboratory Processing Instructions:
Test performed in BJH Flow Cytometry.