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Test Code LAB5623 Everolimus, Blood

Methodology

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Specimen Requirements

Specimen Type: Whole blood
Container/Tube: Lavender top (EDTA)-Separator gel tube is not acceptable.
Specimen Volume: Full tube

 

Collection Instructions:

Do not centrifuge.

1. Draw specimen for trough level immediately prior to next dose.

Specimen Transport Temperature

Ambient

 

Reference Values

Results are reported in ng/mL.
The therapeutic range for everolimus can vary by transplant organ type and sample timing but a trough range of 3 - 8 ng/mL is typical.

Day(s) Test Set Up

Monday through Sunday
Cut-off time: 2330, 0600, 0900, 1200, 1530
Turnaround Time:

STAT: not available

Routine: 3 hours after cut-off time

Test Classification and CPT Coding

80169

Additional Information

For BJH Laboratory Use Only

Analyte Stability:

  • Specimens must be processed within 7 days.
  • Processed sample stability:
    • Ambient - 7 days
    • Refrigerated (2 - 8°C) - 7 days
    • Frozen (≤ -20°C) - 2 weeks

Mix freshly collected, refrigerated, or thawed specimen thoroughly prior to use. Avoid repeated freezing and thawing.

  • See Analyte Stability Chart under General Information for a complete list.

Laboratory Processing Instructions:
Test performed in BJH Core Lab.  Log in and place in the pending specimen refrigerator. Do not centrifuge. Do not freeze. Completed specimen is stored refrigerated for 5 days.

LOINC Code Information

Name LOINC Code
Everolimus 50544-6
Everolimus Trough 77349-9