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Test Code LAB5029 (T); LAB145 (R); LAB5028 (P) Amikacin Level

Important Note

***IMPORTANT NOTE***

Christian Hospital Laboratory:  This test is to be processed and sent as a stat to performing laboratory, (ie. Barnes Jewish Hospital, St. Louis Children's Hospital)

Performing Laboratory

St. Louis Children's Hospital

Specimen Requirements

Specimen Type:

 Blood, plasma

Container/Tube

 

Preferred:

Mint green-top lithium heparin (MNTGRN LiHep) Vacutainer®

 

Acceptable:

Plain, red-top Vacutainer® or Lavender-top (EDTA) Vacutainer ®

 

Collection Volume:

2.0 mL (minimum 1.0 mL)

 

Patient Preparation:

None

 

Sample Rejection

Mislabeled or unlabeled specimens.

 

Special Instructions:

Trough Specimen: Collect immediately before next scheduled dose or no earlier than 1 hour prior to dose. (Trough level for first dose should be collected just prior to next dose).

Peak Specimen: Collect 30-60 minutes after dose. 

Send to lab promptly.

Reference Values

Therapeutic Range: ranges apply to all ages

 

Trough Level:

<8.0 mcg/mL

Random Level:

No established reference interval

Peak Level:

10.0-35.0 mcg/mL
     
Critical Values: Random level: ≥75.0 mcg/mL
  Trough level: >8.0 mcg/mL
 

Critical test results will be automatically called back initially and every 24 hours thereafter.

 

CPT Coding

CPT Codes:

80150

LOINC Value:

Amikacin Tr: 3321-7

Amikacin Rnd: 35669-1

Amikacin Pk: 3319-1

Specimen Transport Temperature

Lab Processing instructions:

Centrifuge specimen to separate. If specimen is collected in a plain, red-top or Lavender-top (EDTA) tube, aliquot sample into a Roche false-bottom tube for testing (labeling with specimen type).

 

Processed Volume:

0.3 mL plasma/serum

 

Analyte Stability:

Ambient - unprocessed: 8 hours

PROCESSED:

Ambient: 8 hours

Refrigerated: 2 days

Frozen: 4 weeks

Methodology

Immunoassay (Kinetic interaction of microparticles in solution (KIMS) as measured by changes in light transmission).

 

Day(s) Test Set Up

Test Availability:

Monday-Sunday

Cut-off time:

None; performed as received

Turnaround Time:

Same day; 2 hours from receipt in lab