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Test Code LAB311 Antithrombin Activity, Blood

Methodology

Chromogenic

Literature References:

Package insert:   HemosIL Liquid Antithrombin  Kit (pn20030100) Instrumentation Laboratory, issued 08/2014.

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Specimen Requirements

Specimen Type: Blood

Container/Tube: Light-blue top (citrate) tube.

Specimen Volume: Full tube

Specimen Minimum Volume: Full tube

Collection Instructions:

  1. Draw a discard blue top tube prior to drawing a light-blue top (citrated) tube if using a Winged Infusion Set.  Discard tube should be under filled and discarded.
  2. Clotted blood or an insufficient specimen is not acceptable.
  3. Avoid phlebotomy techniques which cause hemolysis and heparin contamination.
  4. Follow nursing policy on volume of blood to discard prior to collecting blood from indwelling heparinized catheters.


Analyte Stability:
4 hours at 18° C to 24° C.

Platelet-free plasma can be frozen at -20 for transport on dry ice up to 72 hours
or stored 6 months at -70° C.


Additional Information:

If the patient is a hospital patient and the hematocrit is >55%, call Barnes-Jewish Hematology Laboratory at 314-362-5203 for instructions.

Specimen Transport Temperature

Ambient Yes/Refrigerate NO/Frozen Yes

Reference Values

80%-125%

Literature References:

1. Egeberg O. Inherited antithrombin deficiency causing thrombophilia. Thromb Diath Haemorrh 1965;13:516-30.
2. Fareed J, Messmore HL, Walenga JM, et al. Laboratory evaluation of antithrombin III: a critical overview of currently available methods for antithrombin III measurements. Semi Thromb Hemost 1982;8(4):288-301.

Day(s) Test Set Up

Monday through Sunday

Turnaround Time:
STAT: 1 hour after receipt in laboratory

Routine: 2-4 hours after receipt in laboratory

Test Classification and CPT Coding

85300

Additional Information

For BJH Laboratory Use Only
Analyte Stability:

4 hours at 18° C to 24° C.

Platelet-free plasma can be frozen at -20 for transport on dry ice up to 72 hours
or stored 6 months at -70° C.

Laboratory Processing Instructions:
Test performed in BJH Core Lab.

LOINC Code Information

Name LOINC Code
Antithrombin Activity, Blood 3174-0