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HelpTest Code LAB2900 Hemoglobin Analysis, Blood
Methodology
Capillary Electrophoresis/Gel Electrophoresis
Testing Algorithm:
On all first time patients, for hemoglobins that appear in the S or C position, confirmatory testing by sickle cell test acid gel electrophoresis will be performed, at an additional charge. For all other hemoglobins, confirmation testing by acid and alkaline gel electrophoresis will be performed, at an additional charge.
Performing Laboratory
Barnes-Jewish Hospital Laboratory
Specimen Requirements
Specimen Type: Whole blood
Container/Tube: Lavender top (EDTA)
Specimen Volume: Full tube
Specimen Transport Temperature
Ambient
Reference Values
Hemoglobin A1: 96.0%-98.5%
Hemoglobin A2: 1.5%-3.2%
Hemoglobin F: ≤1.0%
Literature References:
1. Hoyer JD, Kroft SH. Color atlas hemoglobin disorders: a compendium based on proficiency testing. College of American Pathologist, 2003.
2. Giordano, PC. Carrier diagnostics and prevention of hemoglobinopathies using capillary electrophoresis. Sebia Laboratory, 2007.
Day(s) Test Set Up
Tuesday and Thursday
Turnaround Time:
STAT: not available
Routine: 2-5 days
Test Classification and CPT Coding
83020-Capillary electrophoresis
83020-Acid hemoglobin electrophoresis (if appropriate)
83020-Alkaline hemoglobin electrophoresis (if appropriate)
Additional Information
For BJH Laboratory Use Only
Analyte Stability:
7 days refrigerated
Laboratory Processing Instructions:
Test performed in BJH Core Lab. RBC profile completed on XN. Refrigerate specimen until assayed on Capillarys..
LOINC Code Information
| Name | LOINC Code |
| Acid Hemoglobin Electrophoresis | 13515-2 |
| Alkaline Hemoglobin Electrophoresis | 49323-9 |
| Hemoglobin A | 20572-4 |
| Hemoglobin A2 | 27345-8 |
| Hemoglobin C | 32681-9 |
| Hemoglobin F | 32682-7 |
| Hemoglobin S | 32683-5 |
| Hemoglobin Analysis Interpretation | 20492-5 |