Test Code LAB196 Protein, Body Fluid
Methodology
Colorimetric (Biuret)
Literature Reference:
Package insert: Total protein reagent. Roche Corporation, 10/2018.
Performing Laboratory
Barnes-Jewish Hospital Laboratory
Specimen Requirements
Specimen Type: Pleural fluid, Peritoneal Fluid, Pericardial Fluid
Container/Tube: Mint Green top tube
Specimen Volume: 2 mL
Additional Information: Indicate fluid type on specimen container.
Specimen Transport Temperature
Ambient
Reference Values
Ratio of pleural to serum protein ≥ 0.5 is indicative of exudate and <0.5 of transudate.
Peritoneal protein ≥ 3.0 g/dL is indicative of exudates and <3.0 g/dL of transudates.
Ratio of pericardial to serum protein ≥ 0.5 is indicative of exudate and <0.5 of transudate.
Literature References:
1. Block, Darci R. Tietz Textbook of clinical Chemistry and Molecular Diagnositics. Elsevier Sixth Edition 2017. Body Fluids Abstract p. 925
2. Yataco, Jose C, Cleveland Clinic Journal of Medicine. Pleural effusions: Evaluation and Management. October 2005. Volume 72, Number 10.
Day(s) Test Set Up
Monday through Sunday
Turnaround Time:
STAT: 1 hour after receipt in laboratory
Routine: 2 to 4 hours after receipt in laboratory
Test Classification and CPT Coding
84157
Additional Information
For BJH Laboratory Use Only
Alternate Tube Type: Red top tube
Analyte Stability:
See Analyte Stability Chart under General Information for a complete list.
Laboratory Processing Instructions:
Test performed in BJH Core lab. If specimen is received in a VACUTAINER, check procedure to confirm suitability. Centrifuge upon receipt. Apply blue tape to specimen aliquot tube. Deliver to the Cobas 8000. Completed specimen is stored frozen for 30 days.
LOINC Code Information
Fluid Type |
Loinc code |
Pleural Fluid |
2882-9 |
Peritioneal Fluid |
2883-7 |
Pericardial Fluid |
33407-8 |