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Test Code LAB182 Bilirubin, Total, Body Fluid

Performing Laboratory

Barnes-Jewish Laboratory

Methodology

Colorimetric (Diazo Jendrassik-Grof)

 

Literature Reference: Package insert: Total Bilirubin reagent. Roche Corporation BLT3, 01/2020.

 

 

Specimen Requirements

Specimen Type: Pleural fluid, Ascites fluid, J-P fluid

Container/Tube: Mint green

Specimen Volume: 2mL

 

Indicate fluid type on specimen container

Day(s) Test Set UP

Monday through Sunday

 

Turnaround Time

Stat: 1 hour after receipt in laboratory

Routine: 2 to 4 hours after receipt in laboratory

Reference Values

Ratio of pleural to serum Bilirubin ≥ 0.6 is indicative of exudate and <0.6 of transudate.

Ratio of peritoneal fluid to serum Bilirubin ≥ 0.6 is indicative of exudate and <0.6 of transudates. Peritoneal fluid minus serum Bilirubin gradient >5 mg/dL suggests bile duct leakage into peritoneal fluid.

Literature References:

DeBenedet et al. Scheiman JM, Elta GH, Elmunzer BJ. Peritoneal fluid bilirubin to serum bilirubin ratio for the diagnosis of bile leaks in orthotopic liver transplant recipients. Dig Dis Sci. 2013 Oct; 58(10):3044-8.

ARUP Test directory, Body Fluid Reference Intervals and/or Interpretative Information.

 

Test Classification and CPT Coding

82247

Additional Information

For BJH Laboratory Use Only

 

Alternate Tube Type: Red top tube

 

Analyte Stability: 

See Analyte Stability Chart under General Information for a complete list.

 

Laboratory Processing Instructions: Test performed in BJH Core lab. If specimen is received in a VACUTAINER, check procedure to confirm suitability. Centrifuge upon receipt. Apply blue tape to specimen aliquot tube. Deliver to the Cobas 8000 for analysis. Completed specimens are stored frozen for 30 days.

LOINC Code Information

 

Fluid Type

Loinc code

Pleural Fluid

14421-2

Ascites Fluid

14422-0