Test Code LAB178 Amylase, Body Fluid
Methodology
Enzymatic
Literature Reference:
Package insert: Amylase AMYL2 reagent. Roche Corporation, 12/2019.
Performing Laboratory
Barnes-Jewish Hospital Laboratory
Specimen Requirements
Specimen Type: Pleural fluid, Ascites fluid, J-P fluid, pancreatic cyst fluid
Container/Type: Mint Green tube
Specimen Volume: 2 mL
Additional Information: Indicate fluid type on specimen container.
Specimen Transport Temperature
Ambient
Reference Values
Ratio of pleural to serum amylase >1.0 is indicative of pancreatic excretions in the pleural fluid.
Ascites Fluid amylase is normally equivalent to serum levels, but elevated levels are indicative of pancreatitis or pancreatic secretions.
Ratio of JP fluid to serum amylase >3.0 is indicative of pancreatic fistula.
Pancreatic cyst fluid amylase <250 U/L is indicative of benign serous cyst.
Literature References:
Block, Darci R. Tietz Textbook of clinical Chemistry and Molecular Diagnositics. Elsevier Sixth Edition 2017. Body Fluids Abstract p. 925
ARUP Test directory, Body Fluid Reference Intervals and/or Interpretative Information.
Day(s) Test Set Up
Monday through Sunday
Turnaround Time:
STAT: 1 hour after receipt in laboratory
Routine: 2 to 4 hours after receipt in laboratory
Test Classification and CPT Coding
82150
Additional Information
For BJH Laboratory Use Only
Alternate Tube Type: Red top tube
Analyte Stability:
See Analyte Stability Chart under General Information for a complete list.
Laboratory Processing Instructions:
Test performed in BJH Core lab. If specimen is received in a VACUTAINER, check procedure to confirm suitability. Centrifuge upon receipt. Apply blue tape to specimen aliquot tube. Deliver to the Cobas8000 for analysis. Completed specimens are stored frozen for 30 days.
LOINC Code Information
Fluid Type |
Loinc code |
Pleural Fluid |
1796-2 |
Ascites Fluid |
1797-0 |
J-P Fluid |
43816-8 |
Pancreatic Cyst Fluid |
43816-8 |