Test Code 4732-SA HLA: Single Antigen Antibody Screen, Blood

Methodology

Flow Cytometry Based

Note: HLA antibody identification may be performed as Class ISA ID only, Class IISA ID only, or Class I and II.

Barnes-Jewish Hospital Laboratory

Specimen must arrive within 24 hours of draw.

Specimen Type: Whole blood

Container/Tube: 10 mL Red/gray top (SST)

Specimen Volume: ADULT/CHILDREN -Full tube (10 mL) INFANT- 2-3 mL minimum

Additional Information:

1. Do not refrigerate.

2. Renal dialysis patients should always be drawn before dialysis procedure.

Ambient/Refrigerate NO/Frozen NO

Normal: negative
A positive result indicates the presence of IgG anti-HLA antibodies to either Class I or Class II antigens.

Results are reported as percent positive reactions.

Antibodies identified are reported.

Monday through Friday

Turnaround Time:
STAT: 48 hours. (Call HLA Laboratory at 314-362-5322 to request STAT)
Routine: 1 week

For BJH Laboratory Use Only
Alternate Tube:

Red top tubes

Gold top tubes (SST)

Laboratory Processing Instructions:
Test performed in BJH HLA Laboratory.

86832

86833