Sign in →

Test Code T&B CEL T- and B-Cell Lymphocyte Profile

Performing Laboratory

NRL-Molecular Diagnostics

Methodology

Flow Cytometry

Useful for quantitation of peripheral blood lymphocyte subsets in immunodeficiency diseases, primarily HIV infection.

Note: WBC count is only performed if WBC and lymphocyte count is not available.

Specimen Requirements

Specimen must arrive within 24 hours of draw.

 

ACD and EDTA whole blood are required.

 

Specimen Type: ACD whole blood

Container/Tube: Yellow top (ACD)

Specimen Volume: 6 mL

Collection Instructions: Label specimen as ACD whole blood.

Additional Information: Draw date, WBC count, and percent lymphocyte counts are required.

 

Specimen Type: EDTA whole blood
Container/Tube: Lavender top (EDTA)
Specimen Volume: 4 mL
Collection Instructions: Label specimen as EDTA whole blood.

Additional Information: Draw date, WBC count, and percent lymphocyte counts are required.

Reference Values

WBC: 5.0-10.0 K/mm3
Lymphocytes %: 16.0-52.0%
Lymphocytes #: 1,200-3,500 cells/µL
Total T-cells (CD3) %: 60-85%
Total T-cells (CD3) #: 720-2,975 cells/µL
Helper T-cells (CD4) %: 29-59%
Helper T-cells (CD4) #: 348-2,065 cells/µL
Suppressor T-cells (CD8) %: 12-43%
Suppressor T-cells (CD8) #: 144-1,505 cells/µL
B-cells (CD 19) %: 7-23%
B-cells (CD19) #: 84-805 cells/µL
Natural killer cells (CD16 + CD56%): 6-29%
Natural killer cells (CD16 + CD56) #: 72-1,015 cells/µL
CD4:CD8 ratio: 0.7-3.5

Day(s) Test Set Up

Monday through Friday (must arrive by noon on Friday)

Turnaround Time:

24 hours

Specimen Transport Temperature

Ambient/Refrigerate NO/Frozen NO

Test Classification and CPT Coding

86064-B cells, total count

86359-T cells, total count

86360-Absolute CD4 and CD8 count, including ratio

86379-Natural killer (NK) cells, total count

85048-WBC (if appropriate)