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Test Code LAB9772 UGT1A1-28 Genotyping for Irinotecan Toxicity

Methodology

Polymerase Chain Reaction (PCR) amplification of the promoter of UGT1A1 is performed with fluorescently-tagged analyte specific reagent primers as described (Shulman K, 2011). PCR Products are separated by capillary electrophoresis.

 

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Specimen Requirements

Call Barnes-Jewish Hospital Molecular Diagnostics Laboratory at 314-454-8685 for collection instructions.

Form: Molecular Diagnostics Requisition

 

Specimen Type: Whole blood

Container/Tube: Lavender top (EDTA)

Specimen Volume: Full tube

Specimen Minimum Volume: 1-2 mL

Collection Instructions: Invert several times to mix blood. Clotted blood is not acceptable.

Specimen Transport Temperature

Ambient/Refrigerate/Frozen NO

Reference Values

An interpretive report will be provided.

Literature Reference:

Innocenti F, Undevia SD, Lyer L, et al. Genetic variants in the UDP-glucuronasyltransferase 1A1 gene predict the risk of severe neutropenia of irinotecan. J Clin Oncol 2004;22(8):1382-88.

Day(s) Test Set Up

Friday

Sample must be received in the laboratory by Thursday at 1600
Turnaround Time:
STAT: Director approval required
Routine: 2 to 7 business days

Test Classification and CPT Coding

81350 

Additional Information

For BJH Laboratory Use Only

Analyte Stability:

Blood is stable 0 to 4 hours at ambient temperature, up to 168 hours refrigerated.

Laboratory Processing Instructions:

Test performed in BJH-Molecular Diagnostics.