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Test Code LAB9767-STR Comprehensive Testing(Recipient); LAB9768-STR Donor Testing STR Comprehensive Testing

Methodology

Short tandem repeat (STR) polymorphic DNA markers are amplified by polymerase chain reaction (PCR) using the Promega PowerPlex 16 HS System, labeled with fluorescent markers and used to distinguish patient and donor cells as different on a Capillary Electrophoresis Fluorescence Detection instrument.

STR markers used in analysis include: D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, Amelogenin, vWA, D8S1179, TPOX, and FGA. (PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)

References

PowerPlex16 HS System Technical Manual. Promega Corporation, 6/2012 revision

 

Van Deerlin VMD. Identity testing: Applications of DNA typing Anatomic Pathology. In Diagnostic Molecular Pathology, Leonard DGB, editor; 2002 Elsevier Science, Philadelphia, PA.

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Specimen Requirements

Call Barnes-Jewish Hospital Molecular Diagnostics Laboratory at 314-454-8685 for collection instructions.

 

Form: Molecular Diagnostics Requisition


Submit only 1 of the following specimens:

 

Specimen Type: Peripheral blood

Container/Tube: Lavender top (EDTA)

Specimen Volume: 2 mL 

Specimen Minimum Volume: 1 mL

Collection Instructions: Invert several times to mix. Clotted blood is not acceptable.

Additional Information: Specimen cannot be frozen.

 

Specimen Type: Bone marrow

Container/Tube: Lavender top (EDTA)

Specimen Volume: 2 mL 

Specimen Minimum Volume: 1 mL

Collection Instructions: Invert several times to mix bone marrow. Clotted bone marrow is not acceptable.

Additional Information: Specimen cannot be frozen.

 

Specimen Type: Cord blood, anticoagulated

Container/Tube: Lavender-top (EDTA)

Specimen Volume: 2 mL

Specimen Minimum Volume: 1 mL

Collection Instructions: Invert several times to mix cord blood. Clotted cord blood is not acceptable.

Additional Information: Specimen cannot be frozen.

 

Specimen Type: Buccal swabs

Call Barnes-Jewish Hospital Molecular Diagnostics Laboratory at 314-454-8685 for details about collection system and shipment. DNA, sufficient for PCR-based assays, may be isolated from cells obtained by a buccal swab; this is particularly well tolerated by children.

Specimen Transport Temperature

All specimen typesshould be sent at ambient or refrigerated temperatures.  

Reference Values

An interpretative report will be provided.

Literature Reference:

Au WY, Chan EC, Lie AKW, et al. Poor engraftment after allogeneic bone marrow transplantation: role of chimerism analysis in treatment and outcome. Ann Hematol 2003;82:410-15.

Day(s) Test Set Up

Monday and Wednesday
Turnaround Time:
STAT: not available
Routine: 3 to 5 business days

Test Classification and CPT Coding

81265 

Additional Information

For BJH Laboratory Use Only
Alternate Tube:

1 yellow-top ACD (solution A or B) tube

Analyte Stability:

Blood and bone marrow are stable 0 to 4 hours at ambient temperature, and up to 168 hours refrigerated.

Laboratory Processing Instructions:

Test performed in BJH Molecular Diagnostics. Please deliver specimens no later than Friday at 1600 for Monday testing and Tuesday at 1600 for Wednesday testing.