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Test Code LAB7872 (L/L phenotype) Flow Cytometry/ Leukemia/Lymphoma Assessment

Important Note

The laboratory will triage the samples and run panels accordingly.  All samples will be checked for viability and cellularity.  They will be canceled QNS if found to be inadequate.

 

In all cases a patient history including diagnosis and a copy of a recent CBC with differential will be most helpful.

 

 

Additional Codes

If quantitative testing is desired, a CD4, CD4/CD8, or a T&B Cell panel can be ordered.  These will only be performed on whole blood samples collected in lavender and yellow top tubes (both are requested for testing).  It is preferable to have samples < 24 hours old.

Performing Laboratory

NRL-Molecular Diagnostics

Methodology

Flow Cytometry

Includes flow cytometric analysis with a panel of lymphocyte- and/or myeloid-lineage associated antigens. The appropriate panel is chosen by a Christian Hospital pathologist based on clinical information, specimen type, specimen volume, cellular characteristics, and/or morphologic review. This test also includes review of morphology and morphologic correlation with immunophenotypic results.

Useful for immunophenotyping of blood, body fluids, bone marrow, or tissue of patients with suspected leukemia/lymphoma.

Specimen Requirements

Forms: Christian Hospital Hematopathology-Bone Marrow in Requisition in Special Instructions

Note: Name and telephone number of the ordering physician and a brief history are essential to achieve a consultation fully relevant to the ordering physician’s needs.

 

Specimen must arrive within 24 hours of collection.

 

Whole Blood

Lavender EDTA and Yellow ACD whole blood are required.

Dark Green Sodium Heparin are also acceptable.

 

Specimen Type: EDTA whole blood

Container/Tube: Lavender top (EDTA)

Specimen Volume: 5 mL

Collection Instructions:

1. Draw date is required.

 

 

Specimen Type: ACD Solution whole blood

Container/Tube: Yellow Top

Specimen Volume: 10 mL

Collection Instructions:

1. Draw date is required.

 

Specimen Type: Body fluid
Container/Tube: Plastic container

Specimen Volume: 1 mL

Collection Instructions: Label specimen as body fluid.

Additional Information: Indicate on requisition “for flow cytometric leukemia/lymphoma analysis.”

 

Bone Marrow

EDTA and sodium heparin bone marrow are required. 

 

Specimen Type: EDTA bone marrow

Container/Tube: Lavender top (EDTA)

Specimen Volume: 2-3 mL

Collection Instructions:

1. Collection date is required.

2. Label specimen as EDTA bone marrow.

 

Specimen Type: Sodium heparin bone marrow

Container/Tube: Green top (sodium heparin)

Specimen Volume: 2-3 mL

Collection Instructions:

1. Collection date is required.

2. Label specimen as sodium heparin bone marrow.

 

Specimen Type: Tissue

Container/Tube: Sterile container

Specimen Volume: 1 cm3 or larger biopsy (or as much tissue as is feasible depending on procedure and/or tissue source).

Collection Instructions:

1. Place specimen intact (do not mince) in 15 mL of tissue culture medium (eg, RPMI, or equivalent). Specimen cannot be fixed.

2. Label specimen as tissue.

3. Tissue samples collected on Friday must be received in the laboratory by noon.

Reference Values

An interpretive report will be provided.

Day(s) Test Set Up

Monday through Friday

Turnaround Time:

48 hours

Specimen Transport Temperature

Ambient/Frozen NO-Blood, Body Fluid, Bone Marrow

 

Refrigerate-Tissue specimens

Specimen Requirements Additional Information

Instructions for the Laboratory:

If NetworkReferenceLab receives an inquiry, refer call to the Flow Cytometry Laboratory. Alert flow cytometry staff and/or pathologist of the request and ask them to consult with the caller.

 

 

Test Classification and CPT Coding

 

Test Classification:This test was developed and its performance characteristics determined by Christian Hospital Flow Cytometry & Pathology Departments. St. Louis, MO. It has not been cleared or approved by the U.S. Food and Drug Administration.
CPT Code:

 

88184-Flow cytometry, cell surface, cytoplasmic, first marker

88185-Each additional marker

88187-Pathologist interpretation

88188-Pathologist interpretation

88189-Pathologist interpretation

Triage Panel

88184-First marker

88185 x 8-Each additional marker

88188-Pathologist interpretation

 

Each specimen submitted for flow cytometry analysis is screened with the triage panel, whenever possible.

At the discretion of the pathologist, additional panels or markers may be analyzed when warranted. An interpretive report is provided with an explanation of testing.

Special Instructions