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Test Code LAB5031 Antineutrophil Cytoplasmic Antibodies (ANCA), Serum

Methodology

Indirect Fluorescence

Testing Algorithm:

If ANCA qualitative screen is positive, then LAB5307 Myeloperoxidase Antibody, Serum, LAB5345 Proteinase 3 Antibody, Serum, and ANCA quantitative testing will be performed at an additional charge.

Literature References:

1. Package insert: NOVA Lite ANCA. INOVA Diagnostics, Inc. 6/2003.
2. Henry JB. Clinical diagnosis and management by laboratory methods. 20th ed. Philadelphia; Saunders, 2001:991-3.

3. Savige J, et al. International consensus statement on testing and reporting of antineutrophil cytoplasmic antibodies (ANCA). Am J Clin Path 1999;111:507-13.

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Specimen Requirements

Specimen Type: Serum

Container/Tube: Plain red top

Specimen Volume: Full tube

 

Specimen Transport Temperature

Ambient

Reference Values

Negative
Autoantibodies to neutrophil cytoplasmic antigens (ANCA) are found in sera of patients with Wegener’s granulomatosis (WG). Patients with vasculitis secondary to other diseases such as systemic lupus erythematosus or rheumatoid arthritis may also produce antibodies which react with neutrophils. These antibodies can be distinguished from the above-mentioned antibodies in patients with WG, the frequency of ANCA approaches 85%. Measurement of ANCA is not useful for discrimination between generalized and limited forms of WG, but changes of titer of ANCA suggest relapse in a patient in clinical remission.
Note: Not all specimens positive for pANCA are positive for myeloperoxidase antibodies.

Literature References:

1. Package insert: NOVA Lite ANCA. INOVA Diagnostics, Inc. 6/2003.
2. Henry JB. Clinical diagnosis and management by laboratory methods. 20th ed. Philadelphia; Saunders, 2001:991-3.

3. Savige J, et al. International consensus statement on testing and reporting of antineutrophil cytoplasmic antibodies (ANCA). Am J Clin Path 1999;111:507-13.

Day(s) Test Set Up

Tuesday, Thursday

Cut-off time: 0800

Turnaround Time:
STAT: not available
Routine: Results available the day of testing. Confirmation results available in 5 to 7 days.

Test Classification and CPT Coding

86036-ANCA

83520-PR3 (if appropriate)

83876-MPO (if appropriate)
86037-ANCA titer (if appropriate)

Additional Information

For BJH Laboratory Use Only
Laboratory Processing Instructions:

Test performed in BJH Core Lab. Processed by automated line and aliquot stored refrigerated for batch analysis. Completed specimens are stored refrigerated for 5 days.

LOINC Code Information

Name LOINC Code
Neutrophil Cytoplasmic Ab, Qual 17351-8
Neutrophil Cytoplasmic Ab, Quant 21023-7
Myeloperoxidase Ab 29643-4
Proteinase 3 Ab 29644-2
ANCA Interpretation 17352-6